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pcdna 3 0 control vector  (Addgene inc)


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    Addgene inc pcdna 3 0 control vector
    Pcdna 3 0 Control Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcdna 3 0 control vector/product/Addgene inc
    Average 93 stars, based on 47 article reviews
    pcdna 3 0 control vector - by Bioz Stars, 2026-05
    93/100 stars

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    Roles of TUG1 or miR-26a in neurological deficits in rats with ICH. (A) pcDNA-TUG1 expression in vivo. (B-D) The effects of TUG1 overexpression on mNSS in rats on the 1st (B), 3rd (C), and 7th (D) day after ICH. (E) The expression of miR-26a mimics in rats. (F-H) The effects of miR-26a mimics on mNSS in ICH rats on the 1st (F), 3rd (G) and 7th (H) day after ICH. ***P < 0.001, vs. sham; #P < 0.05, vs. pcDNA-NC; ##P < 0.01, vs. pcDNA-NC; &P < 0.05, vs. mimic-NC; &&P < 0.01, vs. mimic-NC; &&&P < 0.001, vs. mimic-NC.

    Journal: American Journal of Translational Research

    Article Title: TUG1 aggravates intracerebral hemorrhage injury by inhibiting angiogenesis in an miR-26a-dependent manner

    doi:

    Figure Lengend Snippet: Roles of TUG1 or miR-26a in neurological deficits in rats with ICH. (A) pcDNA-TUG1 expression in vivo. (B-D) The effects of TUG1 overexpression on mNSS in rats on the 1st (B), 3rd (C), and 7th (D) day after ICH. (E) The expression of miR-26a mimics in rats. (F-H) The effects of miR-26a mimics on mNSS in ICH rats on the 1st (F), 3rd (G) and 7th (H) day after ICH. ***P < 0.001, vs. sham; #P < 0.05, vs. pcDNA-NC; ##P < 0.01, vs. pcDNA-NC; &P < 0.05, vs. mimic-NC; &&P < 0.01, vs. mimic-NC; &&&P < 0.001, vs. mimic-NC.

    Article Snippet: The design and construction of recombinant plasmids, including TUG1-expressing pcDNA-TUG1 and vector control pcDNA-NC, were done by Genechem (Shanghai, China).

    Techniques: Expressing, In Vivo, Over Expression

    Effects of TUG1 or miR-26a on cerebral angiogenesis in rats with ICH. A. VEGF mRNA levels in ICH rats treated with mimic-NC or miR-26a mimics. B. The expression of VEGF and CD31 in ICH rats injected with miR-26a mimics. Original magnification, × 400. C. VEGF mRNA levels in ICH rats injected with pcDNA-NC or pcDNA-TUG1. D. IHC was performed to detect the expression of VEGF and CD31 in ICH rats injected with pcDNA-TUG1. Original magnification, × 400. ***P < 0.001, vs. sham; &&P < 0.01, vs. mimic-NC; ###P < 0.001, vs. pcDNA-NC.

    Journal: American Journal of Translational Research

    Article Title: TUG1 aggravates intracerebral hemorrhage injury by inhibiting angiogenesis in an miR-26a-dependent manner

    doi:

    Figure Lengend Snippet: Effects of TUG1 or miR-26a on cerebral angiogenesis in rats with ICH. A. VEGF mRNA levels in ICH rats treated with mimic-NC or miR-26a mimics. B. The expression of VEGF and CD31 in ICH rats injected with miR-26a mimics. Original magnification, × 400. C. VEGF mRNA levels in ICH rats injected with pcDNA-NC or pcDNA-TUG1. D. IHC was performed to detect the expression of VEGF and CD31 in ICH rats injected with pcDNA-TUG1. Original magnification, × 400. ***P < 0.001, vs. sham; &&P < 0.01, vs. mimic-NC; ###P < 0.001, vs. pcDNA-NC.

    Article Snippet: The design and construction of recombinant plasmids, including TUG1-expressing pcDNA-TUG1 and vector control pcDNA-NC, were done by Genechem (Shanghai, China).

    Techniques: Expressing, Injection

    TUG1 directly interacts with miR-26a. A. The miR-26a-binding site on TUG1 was analyzed. B. The WT-TUG1 or MUT-TUG1 luciferase reporter activities were analyzed. C. miR-26a expression in ICH rats treated with pcDNA-TUG1. &&&P < 0.001, vs. mimic-NC; ***P < 0.001, vs. sham; ###P < 0.001, vs. pcDNA-NC.

    Journal: American Journal of Translational Research

    Article Title: TUG1 aggravates intracerebral hemorrhage injury by inhibiting angiogenesis in an miR-26a-dependent manner

    doi:

    Figure Lengend Snippet: TUG1 directly interacts with miR-26a. A. The miR-26a-binding site on TUG1 was analyzed. B. The WT-TUG1 or MUT-TUG1 luciferase reporter activities were analyzed. C. miR-26a expression in ICH rats treated with pcDNA-TUG1. &&&P < 0.001, vs. mimic-NC; ***P < 0.001, vs. sham; ###P < 0.001, vs. pcDNA-NC.

    Article Snippet: The design and construction of recombinant plasmids, including TUG1-expressing pcDNA-TUG1 and vector control pcDNA-NC, were done by Genechem (Shanghai, China).

    Techniques: Binding Assay, Luciferase, Expressing

    TUG1 overexpression abolished the roles of miR-26a in the neurobehavioral recovery and the cerebral angiogenesis of ICH rats. (A-C) The effects of TUG1 overexpression on mNSS in rats treated with miR-26a mimics on the 1st (A), 3rd (B) and 7th (C) day after ICH. (D) pcDNA-TUG1 attenuated the promoting effect of miR-26a on VEGF mRNA level. (E) The expression of VEGF and CD31 in ICH rats treated with miR-26a mimics + pcDNA-NC or miR-26a mimics + pcDNA-TUG1. Original magnification, × 400. (F) Western blot of VEGF and CD31 expression in different groups. ##P < 0.01, vs. pcDNA-NC.

    Journal: American Journal of Translational Research

    Article Title: TUG1 aggravates intracerebral hemorrhage injury by inhibiting angiogenesis in an miR-26a-dependent manner

    doi:

    Figure Lengend Snippet: TUG1 overexpression abolished the roles of miR-26a in the neurobehavioral recovery and the cerebral angiogenesis of ICH rats. (A-C) The effects of TUG1 overexpression on mNSS in rats treated with miR-26a mimics on the 1st (A), 3rd (B) and 7th (C) day after ICH. (D) pcDNA-TUG1 attenuated the promoting effect of miR-26a on VEGF mRNA level. (E) The expression of VEGF and CD31 in ICH rats treated with miR-26a mimics + pcDNA-NC or miR-26a mimics + pcDNA-TUG1. Original magnification, × 400. (F) Western blot of VEGF and CD31 expression in different groups. ##P < 0.01, vs. pcDNA-NC.

    Article Snippet: The design and construction of recombinant plasmids, including TUG1-expressing pcDNA-TUG1 and vector control pcDNA-NC, were done by Genechem (Shanghai, China).

    Techniques: Over Expression, Expressing, Western Blot